29698-H08H1
Human PCSK9 / NARC1 (D374Y) Protein (His Tag)
Sino Biological
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DESCRIPTION
A DNA sequence encoding the human PCSK9 (NP_777596.2, with mutation Asp 374 Tyr) (Met1-Gln692) was expressed with a polyhistidine tag at the C-terminus.
DETAILS
- Tag: C-His
- Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
- Source: HEK293 Cells
- Species: Human
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human LDLR mFc (Cat:10231-H05H) at 2 μg/ml (100 μl/well) can bind Human PCSK9(D374Y) His(Cat:29698-H08H1), the EC50 of PCSK9（D374Y) His is 1-20 ng/mL. 2.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag (Cat. No. 10231-H05H) on ProA Biosensor, can bind Recombinant Human PCSK9 (D374Y) Protein, His Tag (Cat. No. 29698-H08H1) with an affinity constant of 2.57nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
- Molecule: PCSK9
- Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4.
- Molecular Mass: The recombinant human PCSK9 consists of 673 amino acids and predicts a molecular mass of 72.5 kDa.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_777596.2
- Predicted N Terminal: Gln 31