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CT026-H0323H

Scientist.com Supplier

Human CD3D & CD3E Heterodimer Protein (ECD)

Sino Biological

DESCRIPTION

A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.

DETAILS

  • Tag: C-human IgG1-Fc & His & C-human IgG1-Fc & Flag
  • Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
  • Source: HEK293 Cells
  • Species: Human
  • Storage: Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: Immobilized SP34 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Human CD3D & CD3E Heterodimer Protein (ECD) (Cat: CT026-H0323H) , the EC50 is 8-25 ng/mL.
  • Category: Proteins
  • Molecule: CD3D & CD3E
  • Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile PBS, pH 7.4.
  • Molecular Mass: The recombinant heterodimer of human CD3D/CD3E consists of 687 (334+353) amino acids and predicts a molecular mass of 77.5 (37.7+39.8) kDa. It migrates as an approximately 44-54 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: P04234-1 & P07766-1
  • Predicted N Terminal: Phe 22 & Asp 23