DESCRIPTION

• Aa: 1-1090
• Mw: 125 kDa
• Tags: C-terminal FLAG-tag
• Format: Aqueous buffer solution
• Genbank: NM_001042603
• Species: Human
• Uniprot: P29375
• Shiptemp: -80°C (dry ice)
• Synonyms: KDM5A, RBBP2, JARID1A
• Warnings: Avoid freeze/thaw cycles.
• Category: Demethylase/Enzymes
• References: 1. Roesch, A., et al., (2005). Mod Pathol. 18(9):1249-57.2. Zhou, X., et al., (2010). Cancer Res. 70(10):4214-213. Van Zutven, LJ., et al., (2006). Genes Chromosomes Cancer 45(5):437-46. Application Reference(s): A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)
• Description: Human JARID1A, also known as KDM5A and RBBP2, GenBank Accession #NM_001042603), a.a. 1-1090 with C-terminal FLAG-tag, MW= 125 kDa, expressed in Sf9 cells via a baculovirus expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JARID1A (80 - 400 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
• Specific Activity: 0.027 pmole/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Demethylase