AAV6 Luciferase-eGFP

DESCRIPTION

Adeno-Associated Virus serotype 6 (AAV6) appears to be related to AAV1 by sequence analysis and shows the highest transduction efficiency in pancreatic beta-cells compared to other AAV serotypes. These AAV6 particles constitutively express the firefly (Photinus pyralis) luciferase and enhanced Green Fluorescent Protein (eGFP) genes connected via a T2A linker, under the control of a CMV promoter. The T2A self-cleaving peptide (derived from Thosea asigna virus 2A) leads to the efficient cleavage of the transcript, and expression of luciferase and eGFP as two separate proteins.

DETAILS

Notes: Biosafety: Recombinant AAV is inherently replication-deficient and not known to cause any human diseases. Additionally, following transduction, AAV vectors exist episomally and do not integrate into or disrupt the host cell's genome. AAV requires the use of a Biosafety Level 1 facility. BPS Bioscience recommends following all local, federal, state, and institutional regulations and using all appropriate safety precautions. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.
Species: Wild-type AAV Serotype 6
Shiptemp: -80°C (dry ice)
Warnings: Avoid freeze/thaw cycles.
Category: Viral Tools/AAV
Description: Adeno-Associated Virus serotype 6 (AAV6) appears to be related to AAV1 by sequence analysis and shows the highest transduction efficiency in pancreatic beta-cells compared to other AAV serotypes. These AAV6 particles constitutively express the firefly (Photinus pyralis) luciferase and enhanced Green Fluorescent Protein (eGFP) genes connected via a T2A linker, under the control of a CMV promoter. The T2A self-cleaving peptide (derived from Thosea asigna virus 2A) leads to the efficient cleavage of the transcript, and expression of luciferase and eGFP as two separate proteins.
Formulation: AAV6 was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68.
Supplied As: Two vials (50 µl x 2) of AAV at a titer ≥1 x 1012 TU/ml. The titer is determined by qPCR and will vary with each lot; the exact value will be provided with each shipment.
Unspsc Code: 41106621
Unspsc Name: Virus mediated expression vectors or kits
Applications: Use as a positive control for transduction Optimize transduction assays and track protein expression over time
Product Type: Viral Tools
Purification: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium #21005-1L). The purity will vary with each lot; the exact value will be provided with each shipment.
Biosafety Level: BSL-1
Related Products: 78448, 78463, 78461, 78465
Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C.
Scientific Category: AAV