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APR-093_25 mcl APR-093_50 mcl APR-093_0.2 ml APR-093-CF_0.2 ml

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Anti-Plexin-A3 (extracellular) Antibody

Alomone Labs

DESCRIPTION

Anti-Plexin-A3 (extracellular) Antibody (#APR-093) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize PLXNA3 from rat, mouse, and human samples.

DETAILS

  • Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
  • Host: Rabbit
  • Clone: NA
  • Label: Unconjugated
  • Purity: Affinity purified on immobilized antigen.
  • Target: PLXNA3, PLXN3, Semaphorin receptor SEX
  • Comment: Contact Alomone Labs for technical support and product customization
  • Gene Id: 18846
  • Isotype: Rabbit IgG
  • Homology: Rat - identical; human - 12/14 amino acid residues identical
  • Is Toxin: No
  • Sequence: (C)KVRVDGSQDAQLYE, corresponding to amino acid residues 440-453 of mouse PLXNA3
  • Synonyms: PLXNA3, PLXN3, Semaphorin receptor SEX
  • Accession: P70208
  • Clonality: Polyclonal
  • Lead Time: 1-2 Business Days
  • Reactivity: Human|Rat|Mouse
  • Formulation: PBS pH7.4
  • Applications: IF|IFC|IHC|LCI|WB
  • Preservative: No Preservative
  • Reconstitution: 0.2 ml double distilled water (DDW).
  • Blocking Peptide: BLP-PR093
  • Negative Control: BLP-PR093
  • Positive Control: NA
  • Cited Application: IHC
  • Immunogen Location: Extracellular, N-terminus
  • Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
  • Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
  • Immunogen Source Species: Mouse
  • Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
  • Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
  • Applications May Also Work In: IF|IFC|IHC|LCI|WB
  • Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
  • Product Page - Scientific Background: Plexins are type 1 transmembrane (TM) receptors acting as key regulatory proteins in a wide variety of developmental, regenerative and pathological processes. They are involved in guidance and motility of vascular, lymphatic vessel, and neuron growth, and in cancer metastasis.Plexin family includes 9 proteins: Plexin-A1-4, -B1-3, -C1 and -D1. Class A Plexins interact with the secreted class 3 Semaphorins, and require Neuropilins (nrps) as co-receptors.Plexin protein signaling results in cytosolic GTPase-activating protein activity including direct interactions with Rho GTPases and transient/catalytic interactions with Ras GTPases1,2.Plexin structure consists of an extracellular semaphorin domain, three Plexin-Semaphorin-Integrin (PSI) domains, and three immunoglobulin, Plexin, and transcription factor (IPT) domains. In addition, a single-spanning transmembrane domain, and a cytosolic region (CYTO) homologous with Ras GTPase-activating proteins (GAPs)2. The transmembrane region of Plexin-A3 is characterized by poly-glycine usually used for close helix-helix packing and aromatic residues that distort helix conformation and association. Plexin-A family members only have 22 residues spanning the membrane1.Mutations in Plexins have been reported in melanoma, lung, breast, pancreatic, and prostate cancers, strongly suggesting that their signaling may play a role in cancer development. Plexins are widely expressed in the nervous system and in the developing cerebral cortex2.
  • Standard Quality Control of Each Lot: Western blot analysis
  • Application Dilutions Western Blot Wb: 1:200
  • Antibody Concentration After Reconstitution: 1 mg/ml
  • Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:200