DESCRIPTION

• Category: REAGENTS
• Keywords: qPCR, PCR, Real Time, Mix, cDNA, Taq, DNA Polymerase, cDNA synthesis, 1-step kit, one-step kit, one step kit, absolute, reverse transcriptase, mRNA, RNA, viral RNA, RT-PCR, RT-qPCR, real-time RT-PCR
• Paragraph 1: qPCRBIO 1-Step SyGreen mix uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The qPCR is performed by PCRBIO Taq HS DNA Polymerase, resulting in robust RT-PCR performance. The 1-step kits can be used to quantify any RNA template inclusing mRNA, total RNA and viral RNA sequences. The 1-step Go kit is designed for extremely fast qPCR and gives the earliest Ct values from high template concentrations.
• Paragraph 2: qPCRBIO 1-Step SyGreen mix can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation. any modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.
• Bullet Point 1: Designed for speed (earliest Ct) and ideally suited to detection from high template concentrations
• Bullet Point 2: Recommended for template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction
• Bullet Point 3: Thermostable reverse transcriptase 45°C to 55°C
• Bullet Point 4: Contains advanced RNase inhibitor
• Bullet Point 5: Non-PCR inhibiting intercalating dye
• Bullet Point 6: Rapid extension rate for early Ct values
• Bullet Point 7: Market leading sensitivity - increased limit of detection
• Bullet Point 8: Compatible on all real-time PCR platforms - standard and fast cycling conditions
• Manufacturer Sku: PB25.32-12