Skip to Main Content
Welcome to the Scientist.com Marketplace

Go to Main Navigation

GLO-H5283-50ug GLO-H5283-500ug

HHV-5 (strain AD169) gH&gL&gO, Twin-Strep Tag&Tag Free&His Tag

ACROBiosystems

DETAILS

  • Tag: C-Twin Strep | Native | C-10×His
  • Host: HEK293
  • Size: 500ug
  • Buffer: PBS, pH7.4
  • Format: Powder
  • Purity: 90%
  • Storage: -20℃
  • Category: Viral Proteins
  • Molecule: gH&gL&gO
  • Conjugate: Unconjugated
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: Human cytomegalovirus (HCMV), a prototypical beta-herpes virus, is a major cause of morbidity and mortality in immunocompromised transplant recipients and congenitally infected fetuses.Different HCMV glycoprotein complexes are capable of meditating entry through interaction with cell-specific receptors on a panel of host cells. The pentamer (gH/gL/UL128/UL130/UL131A) mediates HCMV entry into epithelial, endothelial, and myeloid cells by its binding to neuropilin 2 (Nrp2). The trimer (gH/gL/gO) can mediate infection of all cell types by binding to platelet-derived growth factor-alpha (PDGFRα). Both pentamer and trimer need to interact with the glycoprotein B (gB), the fusogenic protein, to trigger the virus and host cell membrane fusion.
  • Exp Region: Arg 24 - Leu 720 & Val 31 - Arg 278 & Lys 31 - Gln 466
  • Swiss-prot: P12824,P16832,P16750
  • Characteristics: This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 82.6 kDa | 27.6 kDa | 52.6 kDa. The protein migrates as 33 kDa,120-160 kDa and >180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Endotoxin Level: 1.0 EU per μg
  • Molecular Weight: 82.6 kDa & 27.6 kDa & 52.6 kDa
  • Shipping Condition: RT
  • Species Reactivity: HHV-5
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html

DESCRIPTION

HHV-5 (strain AD169) gH&gL&gO, Twin-Strep Tag&Tag Free&His Tag (GLO-H5283) is expressed from human 293 cells (HEK293). It contains AA Arg 24 - Leu 720 & Val 31 - Arg 278 & Lys 31 - Gln 466 (Accession # P12824 & P16832 & P16750).