HHV-5 (strain AD169) gH&gL&gO, Twin-Strep Tag&Tag Free&His Tag

DESCRIPTION

Human cytomegalovirus (HCMV), a prototypical beta-herpes virus, is a major cause of morbidity and mortality in immunocompromised transplant recipients and congenitally infected fetuses.Different HCMV glycoprotein complexes are capable of meditating entry through interaction with cell-specific receptors on a panel of host cells. The pentamer (gH/gL/UL128/UL130/UL131A) mediates HCMV entry into epithelial, endothelial, and myeloid cells by its binding to neuropilin 2 (Nrp2). The trimer (gH/gL/gO) can mediate infection of all cell types by binding to platelet-derived growth factor-alpha (PDGFRα). Both pentamer and trimer need to interact with the glycoprotein B (gB), the fusogenic protein, to trigger the virus and host cell membrane fusion.

DETAILS

Tag: C-Twin Strep | Native | C-10×His
Host: HEK293
Size: 500ug
Buffer: PBS, pH7.4
Format: Powder
Purity: 90%
Source: HHV-5 (strain AD169) gH&gL&gO, Twin-Strep Tag&Tag Free&His Tag (GLO-H5283) is expressed from human 293 cells (HEK293). It contains AA Arg 24 - Leu 720 & Val 31 - Arg 278 & Lys 31 - Gln 466 (Accession # P12824 & P16832 & P16750).
Storage: -20℃
Category: Viral Proteins
Molecule: gH&gL&gO
Conjugate: Unconjugated
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Arg 24 - Leu 720 & Val 31 - Arg 278 & Lys 31 - Gln 466
Swiss-prot: P12824,P16832，P16750
Package Size: 250ug*2
Characteristics: This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 82.6 kDa | 27.6 kDa | 52.6 kDa. The protein migrates as 33 kDa,120-160 kDa and >180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 82.6 kDa & 27.6 kDa & 52.6 kDa
Shipping Condition: RT
Species Reactivity: HHV-5
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