DESCRIPTION

• Mw: 97.5 kDa
• Tag: Myc-DDK
• Accn: NM_001003819
• Type: Human Tagged ORF Clone
• Refseq: NM_001003819.1, NP_001003819.1
• Status: In Stock*
• Symbol: TRIM6-TRIM34
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 445372
• Orf Size: 2526 bp
• Synonyms: IFP1; RNF21; RNF89; TRIM6; TRIM34
• Refseq Orf: 2529 bp
• Refseq Size: 3490 bp
• Availability: In Stock
• Cytogenetics: 11p15.4
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This gene represents a readthrough transcript from genes TRIM6 and TRIM34, and it was described as a splice variant of TRIM34. This gene is mapped to chromosome 11p15, where it resides within a TRIM gene cluster. [provided by RefSeq, Nov 2009]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC223750 representing NM_001003819 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGTGCGGGTCAGAGAGGATTCTACAGGCAGGAAACATCTTAGAAATCAGGGTTGGGCAGGCAGGAGCCA GGAGAGTAGCTACAATGACTTCACCAGTACTGGTGGACATACGAGAAGAGGTGACCTGCCCTATCTGCCT GGAGCTCCTAACAGAACCCCTGAGCATAGACTGTGGCCACAGCTTCTGCCAAGCCTGCATCACACCAAAT GGCAGGGAATCAGTGATTGGTCAAGAAGGGGAAAGAAGCTGCCCTGTGTGCCAGACCAGCTACCAGCCAG GGAACCTGCGGCCTAATCGGCATCTGGCCAACATAGTGAGGCGGCTCAGAGAGGTAGTGTTGGGCCCTGG GAAGCAGCTGAAAGCAGTTCTTTGTGCAGACCATGGAGAAAAACTGCAGCTCTTCTGTCAGGAGGATGGG AAGGTCATTTGCTGGCTTTGTGAGCGGTCTCAGGAGCACCGTGGTCACCACACGTTCCTCGTGGAGGAGG TTGCCCAGGAGTACCAGGAGAAGTTTCAGGAGTCTCTAAAGAAGCTGAAGAACGAGGAGCAGGAAGCTGA GAAGCTAACAGCTTTTATCAGAGAGAAGAAGACATCCTGGAAGAATCAGATGGAGCCTGAGAGATGCAGG ATCCAGACAGAGTTTAATCAGCTGCGAAATATCCTAGACAGAGTGGAGCAACGGGAGCTGAAAAAGCTGG AACAGGAAGAGAAGAAGGGGCTACGAATTATAGAAGAGGCTGAGAATGATCTGGTCCACCAGACCCAGTC GCTGCGAGAGCTCATCTCGGATCTGGAGCGTCGATGTCAGGGGTCAACAATGGAGCTGCTGCAGGATGTG AGTGATGTCACAGAAAGGAGTGAGTTCTGGACCCTGAGGAAGCCAGAAGCTCTCCCTACAAAGCTGAGAA GTATGTTCCGAGCCCCAGATCTGAAAAGGATGCTGCGAGTGTGTAGAGAGCTGACAGATGTCCAAAGCTA CTGGGCCATCCAGGGGTCTTTAACCAGAAGAGAGAGGAGAGCCTCAGGAGTTAGGACCAGAAGAAGCCAG GGAAGCAGTGCAATGGCTTCAAAAATCTTGCTTAACGTACAAGAGGAGGTGACCTGTCCCATCTGCCTGG AGCTGTTGACAGAACCCTTGAGTCTAGACTGTGGCCACAGCCTCTGCCGAGCCTGCATCACTGTGAGCAA CAAGGAGGCAGTGACCAGCATGGGAGGAAAAAGCAGCTGTCCTGTGTGTGGTATCAGTTACTCATTTGAA CATCTACAGGCTAATCAGCATCTGGCCAACATAGTGGAGAGACTCAAGGAGGTCAAGTTGAGCCCAGACA ATGGGAAGAAGAGAGATCTCTGTGATCATCATGGAGAGAAACTCCTACTCTTCTGTAAGGAGGATAGGAA AGTCATTTGCTGGCTTTGTGAGCGGTCTCAGGAGCACCGTGGTCACCACACAGTCCTCACGGAGGAAGTA TTCAAGGAATGTCAGGAGAAACTCCAGGCAGTCCTCAAGAGGCTGAAGAAGGAAGAGGAGGAAGCTGAGA AGCTGGAAGCTGACATCAGAGAAGAGAAAACTTCCTGGAAGTATCAGGTACAAACTGAGAGACAAAGGAT ACAAACAGAATTTGATCAGCTTAGAAGCATCCTAAATAATGAGGAGCAGAGAGAGCTGCAAAGATTGGAA GAAGAAGAAAAGAAGACGCTGGATAAGTTTGCAGAGGCTGAGGATGAGCTAGTTCAGCAGAAGCAGTTGG TGAGAGAGCTCATCTCAGATGTGGAGTGTCGGAGTCAGTGGTCAACAATGGAGCTGCTGCAGGACATGAG TGGAATCATGAAATGGAGTGAGATCTGGAGGCTGAAAAAGCCAAAAATGGTTTCCAAGAAACTGAAGACT GTATTCCATGCTCCAGATCTGAGTAGGATGCTGCAAATGTTTAGAGAACTGACAGCTGTCCGGTGCTACT GGGTGGATGTCACACTGAATTCAGTCAACCTAAATTTGAATCTTGTCCTTTCAGAAGATCAGAGACAAGT GATATCTGTGCCAATTTGGCCTTTTCAGTGTTATAATTATGGTGTCTTGGGATCCCAATATTTCTCCTCT GGGAAACATTACTGGGAAGTGGACGTGTCCAAGAAAACTGCCTGGATCCTGGGGGTATACTGTAGAACAT ATTCCCGCCATATGAAGTATGTTGTTAGAAGATGTGCAAATCGTCAAAATCTTTACACCAAATACAGACC TCTATTTGGCTACTGGGTTATAGGGTTACAGAATAAATGTAAGTATGGTGTCTTTGAAGAGTCTTTGTCC TCTGATCCCGAGGTTTTGACTCTCTCCATGGCTGTGCCTCCCTGCCGTGTTGGGGTTTTCCTCGACTATG AAGCAGGCATTGTCTCATTTTTCAATGTCACAAGCCATGGCTCCCTCATTTACAAGTTCTCTAAATGTTG CTTTTCTCAGCCTGTTTATCCATATTTCAATCCTTGGAACTGTCCAGCTCCCATGACTCTATGCCCACCA AGCTCT ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC223750 representing NM_001003819 Red =Cloning site Green =Tags(s) MCGSERILQAGNILEIRVGQAGARRVATMTSPVLVDIREEVTCPICLELLTEPLSIDCGHSFCQACITPN GRESVIGQEGERSCPVCQTSYQPGNLRPNRHLANIVRRLREVVLGPGKQLKAVLCADHGEKLQLFCQEDG KVICWLCERSQEHRGHHTFLVEEVAQEYQEKFQESLKKLKNEEQEAEKLTAFIREKKTSWKNQMEPERCR IQTEFNQLRNILDRVEQRELKKLEQEEKKGLRIIEEAENDLVHQTQSLRELISDLERRCQGSTMELLQDV SDVTERSEFWTLRKPEALPTKLRSMFRAPDLKRMLRVCRELTDVQSYWAIQGSLTRRERRASGVRTRRSQ GSSAMASKILLNVQEEVTCPICLELLTEPLSLDCGHSLCRACITVSNKEAVTSMGGKSSCPVCGISYSFE HLQANQHLANIVERLKEVKLSPDNGKKRDLCDHHGEKLLLFCKEDRKVICWLCERSQEHRGHHTVLTEEV FKECQEKLQAVLKRLKKEEEEAEKLEADIREEKTSWKYQVQTERQRIQTEFDQLRSILNNEEQRELQRLE EEEKKTLDKFAEAEDELVQQKQLVRELISDVECRSQWSTMELLQDMSGIMKWSEIWRLKKPKMVSKKLKT VFHAPDLSRMLQMFRELTAVRCYWVDVTLNSVNLNLNLVLSEDQRQVISVPIWPFQCYNYGVLGSQYFSS GKHYWEVDVSKKTAWILGVYCRTYSRHMKYVVRRCANRQNLYTKYRPLFGYWVIGLQNKCKYGVFEESLS SDPEVLTLSMAVPPCRVGVFLDYEAGIVSFFNVTSHGSLIYKFSKCCFSQPVYPYFNPWNCPAPMTLCPP SS TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Druggable Genome
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin