DESCRIPTION

• Aa: 999-end
• Mw: 67 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_001079817
• Species: Human
• Uniprot: P06213
• Shiptemp: -80°C (dry ice)
• Synonyms: INSR, insulin receptor
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/Receptor Tyrosine
• References: 1. Zhou, P. et al. J. Pediatr. Endocrinol. Metab. 21 (4), 369-375 (2008). 2. Machorro-Lazo, M.V. et al., Gynecol. Obstet. Invest. 67 (1), 14-19 (2008).
• Description: Human insulin receptor (INSR) (GenBank Accession No. NM_001079817), a.a. 999-end with N-terminal GST-tag, MW=67 kDa, expressed in a baculovirus expression system.
• Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, autophosphorylation and phosphorylation of target substrates, screening inhibitors, and selectivity profiling.
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: The enzyme reaction was carried out for 1 hour at room temperature in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 µM of ATP. Substrate : 4 µM Tyr peptide 1 from Invitrogen.
• Related Products: 40242, 101217, 78487
• Specific Activity: 43.3 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase/Receptor Tyrosine