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Recombinant Human IL-27



As a member of the IL-12 family of heterodimeric cytokines that also includes IL-12, IL-23, and IL-35, IL-27 is formed by the association of an IL-27-p28 subunit (also known as IL-30) with the Epstein-Barr Virus (EBV)-induced Gene 3 (EBI3) subunit (also known as IL-27B). Expressed by antigen-presenting cells (APCs) in the early phases of antigen-mediated activation, IL-27 acts as a critical initiator of adaptive immune responses by promoting the rapid clonal expansion of naive CD4+ T cells, IFN-gamma production, and Th1 polarization. IL-27 elicits its effects through receptor complexes IL-27R (also known as TCCR/WSX-1) and gp130, a receptor shared by IL-6. Mainly expressed in monocytes, endothelial cells, and dendritic cells, IL-27 plays an important role alongside IL-6 in the regulation of inflammation and autoimmunity; directly antagonizing IL-6's stimulation of CD4+ T cell proliferation and Th17 differentiation. Recombinant Human IL-27 produced from HEK293 cells is a non-disulfide-linked, heterodimeric protein composed of a 209 amino acid length EBI3 subunit and a 215 amino acid length IL-27-p28 subunit, for a total sequence length of 424 amino acid residues. The calculated molecular weight of the associated IL-27 subunits is 47.8 kDa.


  • Gene Id: Alpha: 246778 Beta: 10148
  • Uniprot: Alpha: Q8NEV9. Beta: Q14213
  • Activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 range is 400-500 ng/ml.
  • Category: RUO Cytokines
  • Subcategory: Growth Factors & Cytokines
  • Research Areas: Immune System, Inflammation, Proliferation
  • Alternative Names: Interleukin-27, Interleukin-27 subunit alpha, IL-27-A, Interleukin-27 subunitbeta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI3, p28, Interleukin-30, IL30
  • Species of Origin: Human
  • Expression System Source: HEK293 cells