Human CD73 / NT5E Protein, His Tag (HPLC-verified) (active enzyme)

DESCRIPTION

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

DETAILS

Tag: C-10×His
Host: HEK293
Size: 500ug
Buffer: 20 mM Tris, 120 mM NaCl, pH7.5
Format: Powder
Purity: 90%
Source: Human CD73 Protein, His Tag (CD3-H52H7) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).
Storage: -20℃
Category: Enzymes
Molecule: CD73
Accession: NP_002517.1
Conjugate: Unconjugated
Stability: ● -20°C to -70°C for 24 months in lyophilized state; ● -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Trp 27 - Ser 549
Swiss-prot: P21589-1
Package Size: 500ug*1
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.9 kDa. The protein migrates as 40 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 59.9 kDa
Shipping Condition: RT
Species Reactivity: Human
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