DESCRIPTION

• Aa: 2-362(end)
• Mw: 42 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_004993
• Species: Human
• Uniprot: P54252
• Shiptemp: -80°C (dry ice)
• Synonyms: Ataxin-3, ATX3, ATXN3, Josephin, JOS, Machado-Joseph disease protein 1, MJD1, Spinocerebellar ataxia type 3, SCA3
• Warnings: Avoid freeze/thaw cycles.
• Category: Ubiquitination/Deubiquitylating Enzymes
• References: 1. do Carmo Costa, M., et al. (2010). PLoS One 5(7):e11728.2. Jung, J., et al. (2009). Hum Mol Genet. 18(24):4843-52. 3. Macedo-Ribeiro, S., et al. (2009). PLoS One 4(6): e5834.
• Description: Human Ataxin-3 (ATX3 or ATXN3), also known as Josephin (JOS), Machado-Joseph disease protein 1 (MJD1) or Spinocerebellar ataxia type 3 (SCA3), GenBank Accession No. NM_004993, a.a. 2-362(end) with an N-terminal His-tag, MW=42.1 kDa, expressed in E. coli.
• Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and Ataxin-3 (0-0.4 ng/µL) in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
• Related Products: 81082
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Ubiquitins: Deubiquitinase (DUB)