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E0083Hu

Human Stromal Cell Derived Factor 1Β, SDF-1B ELISA Kit

Bioassay Technology Laboratory

DESCRIPTION

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed: 29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).

DETAILS

  • GeneID: 6387
  • Species: Human
  • Storage: 2-8ºC
  • MASS(Da): 10,666
  • Synonyms: C-X-C motif chemokine 12;CXCL 12;CXCL12;hIRH;hSDF-1;Intercrine reduced in hepatomas;IRH;PBSF;Pre-B cell growth-stimulating factor;SDF-1;SDF-1-alpha(3-67;SDF-1-beta(3-72;Stromal cell-derived factor 1;
  • Assay Time: 1h 30m
  • Assay Type: Sandwich
  • Gene Names: CXCL12
  • Reactivity: Human
  • Sample Type: Serum, plasma, cell culture supernates
  • Sensitivity: 33.53ng/L
  • Target Protein: CXCL12
  • Detection Range: 70-15000ng/L
  • Full Product Name: Stromal Cell Derived Factor 1 beta
  • Standard Solution: 16000ng/L
  • UniProt Accession: P48061