ZE5-V5220-100ug ZE5-V5220-1mg
Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) Envelope Glycoprotein 1 (GP1) Protein, His Tag
ACROBiosystems
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DESCRIPTION
EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
DETAILS
- Tag: C-8×His
- Host: HEK293
- Size: 1mg
- Buffer: PBS, pH7.4
- Format: Powder
- Purity: 95%
- Source: Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP1, His Tag (ZE5-V5220) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Arg 501 (Accession # AAQ55048.1).
- Storage: -20℃
- Category: Viral Proteins
- Molecule: Glycoprotein / GP (virus)
- Accession: AAQ55048.1
- Conjugate: Unconjugated
- Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
- Exp Region: Ile 33 - Arg 501
- Swiss-prot: P87666.1
- Package Size: 250ug*4
- Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 52.0 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE).
- Endotoxin Level: 1.0 EU per μg
- Molecular Weight: 52.0 kDa
- Shipping Condition: RT
- Species Reactivity: Ebolavirus
- Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html