Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) Envelope Glycoprotein 1 (GP1) Protein, His Tag

DESCRIPTION

EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

DETAILS

Tag: C-8×His
Host: HEK293
Size: 1mg
Buffer: PBS, pH7.4
Format: Powder
Purity: 95%
Source: Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP1, His Tag (ZE5-V5220) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Arg 501 (Accession # AAQ55048.1).
Storage: -20℃
Category: Viral Proteins
Molecule: Glycoprotein / GP (virus)
Accession: AAQ55048.1
Conjugate: Unconjugated
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Ile 33 - Arg 501
Swiss-prot: P87666.1
Package Size: 250ug*4
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 52.0 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 52.0 kDa
Shipping Condition: RT
Species Reactivity: Ebolavirus
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