Lentivirus Purification Mini Kit (10 Preps) Cat. No.:BMG-V1170-01 Size: 10 Preps Description Traditionally the recombinant lentivirus is purified by ultracentrifugation to separate the virus particles from cellular proteins and media components. The ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed, in addition, the ultracentrifugation also concentrates cellular debris, membrane fragments, and unwanted proteins from culture medium. The ViraTrapTM lentivirus mini purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Up to 2x107 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. The viruses are first pelleted from viral supernatant and then further purified and concentrated through a purification column and a desalting/concentration unit. Each column can be regenerated for purifying the same lentivirus. For optimized viral binding and recovery, each column can be regenerated only once.