DESCRIPTION

• Form: Protein G Purified
• Gene: NEFH
• Host: Mouse
• Rrid: AB_2492159
• Type: Primary Antibody
• Clone: NAP4
• Buffer: PBS + 10 mM NaN3.
• Target: Neurofilament NF-H
• Isotype: IgG1
• Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
• Shipping: Blue Ice
• Synonyms: 200 kDa neurofilament protein antibody, CMT2CC antibody, Nefh antibody, Neurofilament heavy polypeptide 200kDa antibody, Neurofilament heavy polypeptide antibody, Neurofilament triplet H protein antibody, NF H antibody, NF-H antibody, NFH antibody, NFH_HUMAN antibody
• Clonality: Monoclonal
• Immunogen: Purified bovine NF-H
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~200 kDa Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
• Applications: WB, IHC, ICC
• Ncbi Gene Id: 4744
• Conjugate Exem: Unconjugated
• Physical State: Liquid
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 200
• Production Notes: Protein G purified tissue culture supernatant.
• Dilution Range Wb: 1:5000
• Immunogen Species: Bovine
• Dilution Range Icc: 1:500-1:1000
• Dilution Range Ihc: 1:500-1:1000
• Species Reactivity: All Known Mammal and Chicken
• Target Description: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3).
• Dilution Range Wb Brain: 1:5000
• Uniprot Number Immunogen Species: P12036

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