VECTASHIELD HardSet Antifade Mounting Medium with Phalloidin, 10 ml. This mounting medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. Fluorescent derivatives of phalloidin are used to stain actin filaments. TRITC (tetramethylrhodamine) is excited at 544 nm and emits at 572 nm, producing an orange-red fluorescence. VECTASHIELD HardSet Mounting Media hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. Features: Ideal refractive index (1.46), Ready-to-use — from the refrigerator to the benchtop, No warming necessary, Can be stored without sealing for long-term analysis, Hardening formulation.