Tissue Proteins Lysis Buffer, 1X Strength Solution: The Tissue Lysis Buffer has been developed for extraction of total soluble protein from animal tissues. The buffer is based on an organic buffer, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the particular application, additional components, such as protease inhibitors, salts, reducing agents or chelating agents may be added to the reagent before proceeding with tissue lysis. The simple composition of this reagent provides versatility and prepared cell lysate may be used for reporter assays (e.g.,luciferase, β-galactosidase, chloramphenical acetyltransferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase),immunoassays (e.g., Western blots, ELISAs, RIAs) and protein purifications. Tissue active protein extraction buffer is mild, contain minimal amount of detergent for cell lysis, and the detergent is dialyzable.