SEK11280
Human CD97 ELISA Pair Set
Sino Biological
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DESCRIPTION
The human CD97 ELISA Pair Set is for the quantitative determination of human CD97.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD97coated on a 96-well plate. Standards and samples are added to the wells, andany CD97 present binds to the immobilized antibody. The wells are washed and ahorseradish peroxidase conjugated mouse anti-CD97 monoclonal antibody is thenadded, producing an antibody-antigen-antibody "sandwich". The wells are againwashed and TMB substrate solution is loaded, which produces color in proportionto the amount of CD97 present in the sample. To end the enzyme reaction, thestop solution is added and absorbances of the microwell are read at 450 nm.
DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 80 ng of recombinant CD97. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 31.25-2000
- Specificity: Human CD97
- Capture Antibody: 0.5 mg/mL of mouse anti-CD97 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 11280-MM07)
- Detection Antibody: 0.5 mg/mL mouse anti-CD97 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11280-MM05)