Human, Mouse and Rat Phospho-NF-kB P65 (Ser536) and Total NF-kB P65 Cell-Based ELISA

DESCRIPTION

RayBio® Human, Mouse and Rat Phospho-NF-kB P65 (Ser536) and Total NF-kB P65 Cell-Based ELISA Kit. This assay semi-quantitatively measures NF-kB P65 phosphorylated at Serine-536 as well as total NF-kB P65 in adherent cell lines.

DETAILS

Alias: Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
Storage: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Category: ELISA
Lead Time: 4 weeks
Assay Type: Direct
Sample Type: Other
Specificity: This ELISA kit recognizes Human, Mouse, Rat NF-kB P65 phosphorylated at site Serine-536 as well as total NF-kB P65.
Subcategory: Cell-based ELISA kits
Result Output: Semi-quantitative
Accession Number: Q04206, Q04207, Q7TQN4
Detection Method: Colorimetric
Species Reactivity: Human, Mouse, Rat