DESCRIPTION

• Mw: 76.5 kDa
• Tag: Myc-DDK
• Accn: NM_001029976
• Type: Human Tagged ORF Clone
• Refseq: NM_001029976.3
• Status: In Stock*
• Symbol: ZNF16
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 7564
• Orf Size: 2046 bp
• Synonyms: HZF1; KOX9
• Refseq Orf: 2049 bp
• Uniprot Id: P17020
• Refseq Size: 2632 bp
• Availability: In Stock
• Cytogenetics: 8q24.3
• Gene Summary: The protein encoded by this gene contains multiple tandem zinc finger motifs. The encoded protein is involved in the differentiation of erythroid and megakaryocytic cells. This gene is located in a cluster of related genes on chromosome 8 encoding zinc finger proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC221669 ORF sequence Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGCCCAGCCTCAGAACTCGCCGTGAGGAGGCAGAGATGGAGCTCTCAGTTCCAGGACCATCCCCCTGGA CCCCTGCAGCCCAGGCCCGTGTGAGAGATGCTCCTGCTGTGACCCACCCTGGATCTGCAGCCTGTGGTAC CCCCTGCTGTAGTGATACTGAGCTGGAAGCCATCTGCCCTCACTATCAGCAGCCAGATTGTGACACCAGG ACTGAAGACAAGGAGTTTCTTCACAAGGAAGACATTCATGAAGATTTGGAATCACAGGCAGAAATATCAG AAAACTATGCTGGTGATGTTTCCCAGGTACCCGAGCTTGGAGATCTGTGTGATGATGTATCAGAAAGAGA CTGGGGAGTCCCCGAAGGCAGGAGGCTGCCACAGTCCCTCTCCCAGGAGGGGGACTTCACACCAGCTGCC ATGGGGCTCCTTAGGGGCCCCTTAGGGGAGAAAGATCTGGACTGTAATGGTTTTGACAGTCGCTTCAGTC TGAGCCCAAACCTGATGGCATGTCAGGAAATCCCTACAGAAGAGAGGCCACATCCATATGACATGGGTGG CCAGAGTTTCCAGCACAGTGTGGACCTAACTGGTCATGAGGGGGTTCCCACAGCTGAAAGTCCACTCATA TGTAATGAGTGTGGGAAAACCTTCCAAGGAAATCCTGACCTTATTCAGCGTCAAATAGTCCACACTGGGG AGGCTTCCTTTATGTGTGATGATTGTGGGAAAACCTTCAGCCAGAACTCAGTTCTTAAAAACCGTCATCG ATCTCATATGAGTGAGAAAGCTTACCAGTGCAGCGAATGTGGGAAAGCCTTCCGAGGGCACTCAGACTTT TCTAGGCATCAGAGTCACCACAGCAGTGAGAGGCCTTATATGTGTAATGAATGTGGAAAAGCCTTCAGCC AGAACTCGAGCCTTAAAAAGCACCAAAAGTCTCACATGAGTGAGAAGCCCTATGAATGCAATGAATGTGG GAAGGCTTTTAGGCGGAGCTCAAACCTCATCCAACATCAAAGAATCCATTCTGGGGAGAAACCGTATGTG TGCAGTGAGTGTGGGAAGGCCTTCAGGCGAAGCTCAAACCTCATCAAACACCACAGGACTCACACAGGAG AGAAGCCTTTTGAGTGTGGCGAGTGTGGGAAAGCCTTCAGCCAGAGTGCACACCTGAGGAAGCACCAGAG GGTCCACACTGGAGAGAAGCCTTATGAGTGTAATGATTGTGGCAAGCCCTTCAGTCGGGTCTCCAACCTC ATTAAGCACCACAGGGTTCACACTGGAGAGAAGCCCTATAAGTGCAGTGACTGTGGGAAAGCATTTAGTC AGAGCTCCAGCCTTATTCAGCATCGGAGAATTCACACTGGAGAAAAGCCTCACGTGTGTAATGTATGTGG AAAAGCCTTTAGTTATAGCTCAGTGCTCCGAAAGCACCAGATCATCCACACGGGAGAGAAGCCGTACAGA TGCAGTGTCTGTGGGAAGGCCTTCAGCCACAGCTCAGCCCTCATTCAGCACCAGGGCGTGCACACAGGCG ACAAGCCCTACGCCTGCCACGAGTGTGGGAAGACCTTTGGTCGCAGCTCCAACCTCATCCTTCACCAGCG AGTCCACACTGGAGAGAAGCCCTATGAATGTACTGAATGTGGAAAAACCTTCAGCCAGAGCTCAACCCTC ATTCAGCATCAGAGGATTCATAATGGGCTGAAGCCCCATGAATGTAACCAGTGTGGTAAAGCCTTCAACC GAAGCTCAAATCTCATTCACCACCAGAAAGTTCATACTGGGGAAAAACCCTACACCTGTGTTGAATGTGG TAAGGGCTTCAGCCAGAGCTCACACCTCATTCAGCATCAGATAATCCACACGGGCGAGCGCCCCTACAAA TGCAGTGAGTGTGGGAAAGCCTTCAGTCAGCGTTCGGTCCTCATCCAGCACCAGAGGATTCACACTGGGG TGAAGCCCTATGACTGTGCTGCTTGTGGGAAAGCCTTCAGCCAGCGATCAAAGTTGATCAAACACCAGTT GATTCACACCAGGGAA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC221669 protein sequence Red =Cloning site Green =Tags(s) MPSLRTRREEAEMELSVPGPSPWTPAAQARVRDAPAVTHPGSAACGTPCCSDTELEAICPHYQQPDCDTR TEDKEFLHKEDIHEDLESQAEISENYAGDVSQVPELGDLCDDVSERDWGVPEGRRLPQSLSQEGDFTPAA MGLLRGPLGEKDLDCNGFDSRFSLSPNLMACQEIPTEERPHPYDMGGQSFQHSVDLTGHEGVPTAESPLI CNECGKTFQGNPDLIQRQIVHTGEASFMCDDCGKTFSQNSVLKNRHRSHMSEKAYQCSECGKAFRGHSDF SRHQSHHSSERPYMCNECGKAFSQNSSLKKHQKSHMSEKPYECNECGKAFRRSSNLIQHQRIHSGEKPYV CSECGKAFRRSSNLIKHHRTHTGEKPFECGECGKAFSQSAHLRKHQRVHTGEKPYECNDCGKPFSRVSNL IKHHRVHTGEKPYKCSDCGKAFSQSSSLIQHRRIHTGEKPHVCNVCGKAFSYSSVLRKHQIIHTGEKPYR CSVCGKAFSHSSALIQHQGVHTGDKPYACHECGKTFGRSSNLILHQRVHTGEKPYECTECGKTFSQSSTL IQHQRIHNGLKPHECNQCGKAFNRSSNLIHHQKVHTGEKPYTCVECGKGFSQSSHLIQHQIIHTGERPYK CSECGKAFSQRSVLIQHQRIHTGVKPYDCAACGKAFSQRSKLIKHQLIHTRE TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Transcription Factors
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin