DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: KMT2B, HRX2, MLL1B, MLL2, MLL4, TRX2, WBP-7, WBP7, CXXC10, lysine methyltransferase 2B, DYT28
• Warnings: Avoid freeze/thaw cycles.
• Category: Methyltransferase/Assay Kit
• References: Dillon, S.C., et al. Genome Biology 2005; 6:227.
• Description: The MLL4 Complex Chemiluminescent Assay Kit is designed to measure MLL4 activity for screening and profiling applications, using purified MLL4 and its complex components: WDR5, RbBP5, Ash2, and DPY30. The key to the MLL4 Complex Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K4 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
• Methodology: Chemiluminescent
• Supplied As: The MLL4 Complex Chemiluminescent Assay Kit comes in a convenient format, with 8-well strips pre-coated with histone H3 peptide substrate, an antibody against methylated lysine on Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methylt
• Unspsc Code: 41105332
• Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
• Applications: Great for studying enzyme kinetics and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 53008, 79670, 79758
• Storage Stability: Up to one year from date of receipt when stored as directed.
• Scientific Category: Methyltransferase
• Instructions for Use: See assay kit datasheet for detailed protocol.