Human GAPDH / G3PDH Protein, His Tag

DESCRIPTION

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is also known as Peptidyl-cysteine S-nitrosylase GAPDH, which is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. GAPDH can participate in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Also, GAPDH can modulate the organization and assembly of the cytoskeleton. Furthermore, GAPDH is a component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.

DETAILS

Tag: N-6×His
Host: E. coli
Size: 1mg
Buffer: 50 mM Tris, 150 mM NaCl, pH7.5
Format: Powder
Purity: 95%
Source: Human GAPDH, His Tag (GAH-H5145) is expressed from E. coli cells. It contains AA Gly 2 - Glu 335 (Accession # AAH01601).
Storage: -20℃
Category: Other Recombinant Proteins
Molecule: GAPDH
Accession: AAH01601
Conjugate: Unconjugated
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Gly 2 - Glu 335
Swiss-prot: P04406
Package Size: 500ug*2
Characteristics: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.9 kDa. The protein migrates as 37-38 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 36.9 kDa
Shipping Condition: RT
Species Reactivity: Human
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