Anti-Actin (Tyr-53), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: ACTB
Host: Rabbit
Rrid: AB_2305190
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Actin (Tyr-53)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Clonality: Rabbit Polyclonal
Immunogen: Phospho-Actin (Tyr-53) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 53 of human β actin. This sequence is identical to similar regions in all four α actins, as well as in γ actin, and is well conserved in actins from most eukaryotic species.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate treated human C2C12 and SYF cSrc transformed cells, but not in control cells. In addition, this antibody either detects actin cleavage products at 36 and 20 kDa, or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 42
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:50
Species Reactivity: Chicken, Human, Mouse, and Rat
Target Description: Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.
Uniprot Number Immunogen Species: P60709
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Tyr-53