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50318-M08H

Mouse CD112 / Nectin-2 / PVRL2 Protein (His Tag)

Sino Biological

DESCRIPTION

A DNA sequence encoding the mouse PVRL2 (NP_033016.3) extracellular domain (Met 1-Gly 351) was fused with a polyhistidine tag at the C-terminus.

DETAILS

  • Tag: C-His
  • Purity: > 98 % as determined by SDS-PAGE
  • Source: HEK293 Cells
  • Species: Mouse
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: Measured by its binding ability in a functional ELISA. 1. Immobilized mouse CD112-His (Cat: 50318-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated mouse DNAM1-His (Cat: 50232-M08H) with a linear range of 0.156-5.0 μg/ml. 2. Immobilized mouse CD112-His (Cat: 50318-M08H) at 10 μg/ml (100 μl/well) can bind mouse DNAM1-Fc (Cat: 50232-M03H) with a linear range of 0.03-1.0 μg/ml.
  • Molecule: NECTIN2
  • Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile PBS, pH 7.4
  • Molecular Mass: The secreted recombinant mouse PVRL2 comprises 331 amino acids with a predicted molecular mass of 36 kDa. As a result of glycosylation, it migrates as an approximately 40-45 kDa band in SDS-PAGE under reducing conditions.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: NP_033016.3
  • Predicted N Terminal: Gln 32