DESCRIPTION

• Aa: 1-211
• Mw: 27 kDa
• Tags: C-terminal His-tag
• Format: Aqueous buffer solution
• Purity: ≥90%
• Genbank: P10415
• Species: Human
• Uniprot: P10415
• Shiptemp: -80°C (dry ice)
• Synonyms: Bcl, Bcl2, B cell lymphoma 2
• Warnings: Avoid freeze/thaw cycles.
• Category: Apoptosis/Protein
• Background: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
• Constructs: BCL-2 (1-211-His)
• References: 1. Labi V. et al., Cell Death Differ. 2008 Jun;15(6):977-87.2. Rong Y.P. et al., Mol Cell. 2008 Jul 25;31(2):255-65.
• Description: Human Bcl-2 (GenBank Accession No. P10415), a.a. 1-211 with C-terminal His-tag, MW = 27 kDa, expressed in an E. coli expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and variable imidazole
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.
• Product Type: Protein
• Host Cell Line: E. coli
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Following the protocol for BPS Cat. #50222. Briefly, BCL-2 was combined with BCL-2 peptide ligand in TR-FRET assay buffer along with anti-His Tb donor and dyelabeled acceptor. Varying concentrations of ABT 199 were added and the reaction was incubated at room temperature for 2 hours followed by fluorescent intensity measurements.
• Related Products: 50222, 100091, 100077, 79601
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Apoptosis