Monoclonal Antibody to CD31 / PECAM-1 (Endothelial Cell Marker)(Clone : 1A10)

DESCRIPTION

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

DETAILS

Gene: PECAM1
App Id: WB,IHC
Antigen: CD31 / PECAM-1 (Endothelial Cell Marker)
Gene Id: 5175
Isotype: Mouse IgG1, kappa
Format Id: T.C. Sup.
Clone Name: 1A10
Uniprot Id: P16284
Host Animal: Mouse
Clonality Id: Monoclonal
Reactivity Id: Human
Product Content: Tissue culture supernatant with 0.05% Azide.
Alternative Name: PECAM1
Application Note: Western Blot (1-2ug/ml);Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of the CD31 (PECAM-1)