Anti-β-Catenin Antibody

DETAILS

Form: Protein A Purified
Gene: CTNNB1
Host: Mouse
Rrid: AB_3068393
Type: Primary Antibody
Clone: M118
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: β-Catenin
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Catenin beta1, CTNNB1, catenin
Clonality: Mouse Monoclonal
Immunogen: Clone (M118) was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 92 kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells. In addition, this antibody recognizes only β-Catenin in immunoprecipitations using anti-β-Catenin versus γ-Catenin.
Applications: WB, ICC, IHC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 92
Dilution Range Ip: 1:100
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:250
Dilution Range Ihc: 1:500
Species Reactivity: Human, Mouse, and Rat
Target Description: β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.
Uniprot Number Immunogen Species: P35222
Post Translational Modification Type: GOmAb