Anti-mDia2 (Thr-1061), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: DIAPH2
Host: Rabbit
Rrid: AB_3068416
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: mDia2 (Thr-1061)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Diap3, Dia2, Drf3, formin
Clonality: Rabbit Polyclonal
Immunogen: Phospho-mDia2 (Thr-1061) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Thr-1061 in mouse mDia2 (Diap3). This peptide sequence is identical in rat and has 2 amino acid differences to human mDia2. The sequence has low homology to other formins.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 135 kDa* protein corresponding to mDia2 in immunoblots of human A7r5 and mouse C2C12 cells treated with calyculin A. The antibody detects transfected mDia2 after treatment with calyculin A, but this reactivity is removed after phosphatase treatment. In addition, the antibody does not detect mDia2 (T1061A) mutant.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 135
Dilution Range Wb: 1:1000
Immunogen Species: Mouse
Species Reactivity: Chicken, Human, Mouse, Rat, and Xenopus
Target Description: Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2). The mDia2 protein has been implicated in cell migration and cytokinesis. This Dia protein can nucleate actin polymerization, as well as bind and stabilize microtubules. mDia2 may also have functions in the nucleus, since it is continually shuttled between the cytoplasm and nucleus
Uniprot Number Immunogen Species: Q9NSV4
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Thr-1061