Monoclonal Antibody to Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)(CALD1/820 + h-CALD)

DESCRIPTION

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

DETAILS

Gene: CALD1
App Id: IHC
Antigen: Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)
Gene Id: 800
Isotype: Mouse IgG1, kappa + Mouse IgG1, kappa
Format Id: Purified
Clone Name: CALD1/820 + h-CALD
Uniprot Id: Q05682
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Affinity Chromatography
Reactivity Id: Human
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: CALD1||CAD||CDM
Application Note: Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Recombinant full-length human CALD1 protein (CALD1/820); Crude human uterus extract (h-CALD)