p104-342t p104-342
Promo Available
Anti-AKT (Thr342) Antibody
PhosphoSolutions
DESCRIPTION
Our Anti-AKT (Thr342) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila AKT (Thr342) and is antigen affinity purified from pooled serum. It is great for use in WB.
DETAILS
- Form: Antigen Affinity Purified from Pooled Serum
- Gene: AKT1
- Host: Rabbit
- Rrid: AB_2492036
- Type: Primary Antibody
- Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
- Target: AKT (Thr342)
- Isotype: IgG
- Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
- Shipping: Blue Ice
- Synonyms: AKT1 antibody, AKT1_HUMAN antibody, AKT2 antibody, AKT3 antibody, PKB alpha antibody, PKB antibody, PKB beta antibody, PKBalpha antibody, PRKBA antibody, PRKBB antibody, PRKBG antibody, Protein kinase B antibody, Protein kinase B beta antibody, Protein kinase B gamma antibody, Proto-oncogene c-Akt antibody, RAC alpha antibody, RAC antibody, RAC gamma antibody, RAC PK alpha antibody, RAC PK beta antibody, RAC PK gamma antibody, RAC-alpha serine/threonine-protein kinase antibody, RAC-PK-alpha antibody, STK2 antibody
- Clonality: Polyclonal
- Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr342 of Drosophila AKT, conjugated to keyhole limpet hemocyanin (KLH)
- Expiration: After date of receipt, stable for at least 1 year at -20°C.
- Specificity: Specific for endogenous levels of the ~68 kDa AKT protein phosphorylated at Thr342. Immunolabeling is completely eliminated with λ-phosphatase treatment. It has been reported that this antibody may also recognize some level of phosphorylated S6K as there is 67% homology with the sequence used as antigen.
- Applications: WB
- Ncbi Gene Id: 41957
- Conjugate Exem: Unconjugated
- Physical State: Liquid
- Quality Control: Western blots performed on each lot.
- Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
- Molecular Weight: 68
- Production Notes: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
- Dilution Range Wb: 1:500
- Immunogen Species: Drosophila
- Species Reactivity: Drosophila
- Target Description: The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (α, β, and γ) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Aktα occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004).
- Uniprot Number Immunogen Species: Q8INB9
- Post Translational Modification Type: Phosphorylated
- Post Translational Modification Residue: Thr342
PROMOTION TERMS
Trial Size Available. Limit 1
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