RC225556
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TCF7 (NM_001134851) Human Tagged ORF Clone
Origene Technologies EU
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DESCRIPTION:
TCF7 (Myc-DDK-tagged)-Human transcription factor 7 (T-cell specific, HMG-box) (TCF7), transcript variant 3
DETAILS
- Mw: 40.61 kDa
- Tag: Myc-DDK
- Accn: NM_001134851
- Type: Human Tagged ORF Clone
- Refseq: NM_001134851.1, NP_001128323.1
- Status: In Stock*
- Symbol: TCF7
- Vector: pCMV6-Entry
- Category: cDNA Clones
- Locus Id: 6932
- Orf Size: 1098 bp
- Synonyms: TCF-1
- Refseq Orf: 810 bp
- Uniprot Id: P36402
- Availability: In Stock
- Cytogenetics: 5q31.1
- Gene Summary: This gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. This gene is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. The encoded protein forms a complex with beta-catenin and activates transcription through a Wnt/beta-catenin signaling pathway. Mice with a knockout of this gene are viable and fertile, but display a block in T-lymphocyte differentiation. Alternative splicing results in multiple transcript variants. Naturally-occurring isoforms lacking the N-terminal beta-catenin interaction domain may act as dominant negative regulators of Wnt signaling. [provided by RefSeq, Oct 2016]
- Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
- Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC225556 representing NM_001134851 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGTACAAAGAGACCGTCTACTCCGCCTTCAATCTGCTCATGCATTACCCACCCCCCTCGGGAGCAGGGC AGCACCCCCAGCCGCAGCCCCCGCTGCACAAGGCCAATCAGCCCCCCCACGGTGTCCCCCAACTCTCTCT CTACGAACATTTCAACAGCCCACATCCCACCCCTGCACCTGCGGACATCAGCCAGAAGCAAGTTCACAGG CCTCTGCAGACCCCTGACCTCTCTGGCTTCTACTCCCTGACCTCAGGCAGCATGGGGCAGCTCCCCCACA CTGTGAGCTGGTTCACCCACCCATCCTTGATGCTAGGTTCTGGTGTACCTGGTCACCCAGCAGCCATCCC CCACCCGGCCATTGTGCCCCCCTCAGGGAAGCAGGAGCTGCAGCCCTTCGACCGCAACCTGAAGACACAA GCAGAGTCCAAGGCAGAGAAGGAGGCCAAGAAGCCAACCATCAAGAAGCCCCTCAATGCCTTCATGCTGT ACATGAAGGAGATGAGAGCCAAGGTCATTGCAGAGTGCACACTTAAGGAGAGCGCTGCCATCAACCAGAT CCTGGGCCGCAGGTGGCACGCGCTGTCGCGAGAAGAGCAGGCCAAGTACTATGAGCTGGCCCGCAAGGAG AGGCAGCTGCACATGCAGCTATACCCAGGCTGGTCAGCGCGGGACAACTACGGGAAGAAGAAGAGGCGGT CGAGGGAAAAGCACCAAGAATCCACCACAGACCCTGGCTCGCCTAAGAAATGCCGTGCTCGCTTTGGCCT CAACCAGCAGACGGATTGGTGTGGTCCGTGCAGGAGGAAAAAGAAATGCATTCGGTACTTACCCGGAGAA GGCCGCTGCCCCAGCCCCGTTCCTTCCGATGACAGTGCTCTAGGCTGCCCCGGGTCCCCAGCTCCCCAGG ACTCACCCTCATACCATCTGCTGCCCCGCTTCCCCACAGAACTGCTTACTAGCCCTGCGGAGCCGGCACC TACATCCCCAGGTCTCTCCACTGCTCTCAGCCTCCCAACCCCAGGGCCCCCACAGGCCCCCCGCAGCACC CTGCAGAGCACACAGGTACAGCAACAGGAATCTCAGAGACAGGTGGCC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC225556 representing NM_001134851 Red =Cloning site Green =Tags(s) MYKETVYSAFNLLMHYPPPSGAGQHPQPQPPLHKANQPPHGVPQLSLYEHFNSPHPTPAPADISQKQVHR PLQTPDLSGFYSLTSGSMGQLPHTVSWFTHPSLMLGSGVPGHPAAIPHPAIVPPSGKQELQPFDRNLKTQ AESKAEKEAKKPTIKKPLNAFMLYMKEMRAKVIAECTLKESAAINQILGRRWHALSREEQAKYYELARKE RQLHMQLYPGWSARDNYGKKKRRSREKHQESTTDPGSPKKCRARFGLNQQTDWCGPCRRKKKCIRYLPGE GRCPSPVPSDDSALGCPGSPAPQDSPSYHLLPRFPTELLTSPAEPAPTSPGLSTALSLPTPGPPQAPRST LQSTQVQQQESQRQVA TR TRPLEQKLISEEDLAANDILDYKDDDDK V
- Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
- Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
- Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
- E Coli Selection: Kanamycin (25 ug/mL)
- Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
- Protein Pathways: Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway
- Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
- Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
- Mammalian Cell Selection: Neomycin