Anti-Caveolin-1 (Tyr-14), Phosphospecific Antibody

DETAILS

Form: Protein A Purified
Gene: CAV1
Host: Mouse
Rrid: AB_2072002
Type: Primary Antibody
Clone: M283
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Caveolin-1 (Tyr-14)
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: caveolin1, vip21
Clonality: Mouse Monoclonal
Immunogen: Clone M283 was generated from a phospho-Caveolin-1 (Tyr-14) synthetic peptide corresponding to amino acids surrounding tyrosine 14 in human Caveolin-1. This sequence has significant homology to the conserved site in rat and mouse Caveolin-1.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of caveolin-1 on SDS-PAGE immunoblots of human A431 cells treated with EGF or pervanadate, and is not observed in untreated cells.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 21
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Human, Mouse, and Rat
Target Description: Caveolins are the primary structural components of the plasma membrane microdomains, caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified, and each has distinct expression patterns. Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis. Caveolins can interact with various signaling molecules, including G-proteins, receptor tyrosine kinases, PKCs, and Src family kinases. Phosphorylation at Tyr-14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, while phosphorylation at Ser-80 regulates caveolin binding to the ER membrane and entry into the secretory pathway.
Uniprot Number Immunogen Species: Q03135
Post Translational Modification Type: GOmAb/Phosphorylated
Post Translational Modification Residue: Tyr-14