DESCRIPTION

• Tag: Tag Free
• Accn: NM_008293
• Type: Mouse Untagged Clone
• Refseq: BC052659, AAH52659
• Status: In Stock*
• Symbol: Hsd3b1
• Vector: PCMV6-Kan/Neo
• Category: cDNA Clones
• Locus Id: 15492
• Synonyms: 3-beta-HSD I; D3Ertd383e
• Refseq Orf: 1122 bp
• Uniprot Id: P24815
• Insert Size: 1122 bp
• Refseq Size: 1612 bp
• Availability: In Stock
• Cytogenetics: 3 42.89 cM
• Gene Summary: A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3-beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein.
• Sequence Data: Fully Sequenced ORF ( show hide ) >BC052659 sequence for NM_008293 AAGAGGTAATCCTCAGCATCCAGACACTCTCATCTGACTTTTAACAATTTAACAGCCCTCCTAAGGGTTA CCCTATATCATACCAGCTCCCACTGTCATCTGTTTCCTGTGTTGACCATGGCTGGATGGAGCTGCCTGGT GACAGGAGCAGGAGGGTTTGTGGGCCAGAGGATCATCAAGATGTTGGTGCAGGAGAAAGAACTGCAGGAG GTCAGAGCTCTGGACAAAGTATTCCGACCAGAAACCAAGGAGGAATTCTCCAAGCTGCAGACAAAGACCA AGGTGACAGTGTTGGAAGGAGACATTCTGGATGCCCAGTGCCTGAGGAGAGCCTGCCAGGGCATCTCTGT TGTCATCCACACTGCTGCTGTCATTGATGTCACAGGTGTCATTCCCAGGCAGACCATCCTAGATGTCAAT CTGAAAGGTACCCAGAACCTATTGGAGGCCTGTGTTCAAGCAAGTGTGCCAGCCTTCATCTTCTGCAGCT CAGTTGATGCTGCAGGGCCCAACTCGTACAAGAAGATCGTCCTGAATGGCCACGAGGAACAGAATCATGA AAGCACATGGTCTGATCCATACCCATACAGCAAAAAGATGGCTGAGAAGGCAGTACTGGCAGCCAATGGG AGCATGCTGAAAAATGGTGGCACTTTGAATACTTGTGCCTTAAGACCCATGTACATTTATGGGGAGAGAA GTCCATTCATTTTTAATGCAATAATTAGGGCCCTCAAAAATAAGGGTATTCTGTGTGTTACTGGCAAATT CTCCATAGCCAACCCAGTATATGTGGAAAATGTGGCCTGGGCACACATTCTGGCAGCCAGGGGCCTTCGA GACCCCAAGAAGTCTACAAGCATCCAAGGACAGTTCTACTACATCTCAGATGACACCCCTCACCAAAGCT ATGATGATTTAAATTACACCCTGAGTAAGGAATGGGGGCTCCGCCCTAATGCCAGCTGGAGCCTTCCTCT GCCCCTGCTCTACTGGCTTGCCTTCCTGCTGGAAACTGTGAGCTTCCTGCTACGTCCAGTCTACAGGTAT AGACCTCTCTTTAACCGCCACTCGATCACACTGTCAAATAGCACGTTCACTTTTTCTTACAAGAAAGCTC AGCGAGATCTGGGCTATGAGCCACTTGTCAACTGGGAGGAAGCAAAGCAGAAAACCTCAGAGTGGATAGG GACAATAGTGGAACAGCACAGGGAGATATTGGACACAAAGTGCCAGTGATGGAAAAAGGGCAGGTACATG GCTCTGGGAGTTATAAGGTCCTGTAGCAAGTACAGAGGCACAAGCCAGGTCCTGCTGTCTCCTGTAGACA CAGAGACCAACTTGGTATCTTTCTCAAGTCACCAGAAGCCTTGCCAGTCACTAACCCACTCACAAACTTG CTTCCCTAAGCCCCTGCTCAGAGACAAACATAGCTGAGTCTCAGTTCTTAGGCTTCAGCAATTACAATTT ACTTTCACTTAGAACTTAGTATTGCTTTTATTTCCCCCTTCAAGTCCTAAATCATATCTTGCCTTTGGTA AAATCCCGTTGCTTTCTGAAGCAAAACAAAAACAGCAATAAATATTGTAATGCCTTAAAAAAAAAAAAAA AA
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: RsrII-NotI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin