Biotinylated Human MR1&B2M Complex Protein (MALS verified)

DESCRIPTION

MR1 is also knwon as MHC class I-related gene protein. Antigen-presenting molecule specialized in displaying microbial pyrimidine-based metabolites to alpha-beta T cell receptors (TCR) on innate-type mucosal-associated invariant T (MAIT) cells. In complex with B2M preferentially presents riboflavin-derived metabolites to semi-invariant TRAV1-2 TCRs on MAIT cells, guiding immune surveillance of the microbial metabolome at mucosal epithelial barriers. Signature pyrimidine-based microbial antigens are generated via non-enzymatic condensation of metabolite intermediates of the riboflavin pathway with by-products arising from other metabolic pathways such as glycolysis. Typical potent antigenic metabolites are 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil (5-OE-RU) and 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), products of condensation of 5-amino-6-D-ribityaminouracil (5-A-RU) with glyoxal or methylglyoxal by-products, respectively.

DETAILS

Tag: C-10×His & C-Avi
Host: HEK293
Size: 25ug
Buffer: PBS, pH7.4
Format: Powder
Purity: 95%
Source: Biotinylated Human MR1&B2M Complex Protein (MRM-H82W5) is expressed from human 293 cells (HEK293). It contains AA Arg 23 - Met 302 (Accession # Q95460-1 (MR1) & P61769-1 (B2M)).
Storage: -20℃
Category: MABSol® Biotin Labeled Proteins
Molecule: MR1 & B2M
Conjugate: Biotin-labeled
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Arg 23 - Met 302
Package Size: 25ug*1
Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 42-45 kDa and 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 36.3 kDa and 11.7 kDa
Shipping Condition: RT
Species Reactivity: Human
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