BRAF (WT) Kinase Assay Kit

DESCRIPTION

BRAF is a serine-threonine kinase activated by the RAS GTPases in response to growth factor stimulation or oncogenic insult. Upon stimulation, BRAF phosphorylates the kinase MEK1 (dual specificity mitogen-activated protein kinase kinase 1), resulting in the activation of the MAP-kinase (mitogen-activated protein) cascade. The MAP-kinase signaling pathway controls cell growth, and dysregulation of this pathway is frequently observed in cancer. Inherited BRAF mutations cause birth defects, while cancer-associated mutations are frequently observed in melanoma. BRAF is a validated therapeutic target for anti-cancer drugs, notably in melanoma. The BRAF (WT) Kinase Assay Kit is designed to measure BRAF(WT) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

DETAILS

Shiptemp: -80°C (dry ice)
Synonyms: BRAF1, RAFB1, B-raf 1, MGC126806, MGC138284, BRAF kit
Warnings: Avoid freeze/thaw cycles.
Category: Kinase/Assay Kit
Description: BRAF is a serine-threonine kinase activated by the RAS GTPases in response to growth factor stimulation or oncogenic insult. Upon stimulation, BRAF phosphorylates the kinase MEK1 (dual specificity mitogen-activated protein kinase kinase 1), resulting in the activation of the MAP-kinase (mitogen-activated protein) cascade. The MAP-kinase signaling pathway controls cell growth, and dysregulation of this pathway is frequently observed in cancer. Inherited BRAF mutations cause birth defects, while cancer-associated mutations are frequently observed in melanoma. BRAF is a validated therapeutic target for anti-cancer drugs, notably in melanoma. The BRAF (WT) Kinase Assay Kit is designed to measure BRAF(WT) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Methodology: Chemiluminescent
Supplied As: The BRAF (WT) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant BRAF enzyme, BRAF substrate, ATP and Kinase Buffer 1 for 100 enzyme reactions.
Unspsc Code: 41105332
Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Product Type: Assay Kit
Biosafety Level: Not applicable (BSL-1)
Related Products: 40533, 40005, 40065, 48688, 78487
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Scientific Category: Kinase
Instructions for Use: See protocol for detailed instructions