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sc-81434 SAMPLE sc-81434

Promo Available Scientist.com Supplier

Akt1/2/3 (5C10)

Santa Cruz Biotechnology

DESCRIPTION

mouse monoclonal IgG1; Akt1/2/3 Antibody (5C10) is an IgG1 mouse monoclonal Akt1/2/3 antibody that detects the Akt1/2/3 protein of mouse, rat, human and canine origin by WB and IP. Akt1/2/3 Antibody (5C10) is available as the non-conjugated anti-Akt1/2/3 antibody. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-βb) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. Activation is dependent on PDGFR-β Tyr residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by Insulin or Insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity. The phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in Insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

DETAILS

  • Host: mouse
  • Type: Primary
  • Uniprot: P31751
  • Clonality: Monoclonal
  • Conjugate: unconjugated
  • Applications: WB, IP
  • Human Gene Id: 207 208 10000
  • Mouse Gene Id: 11651 11652 23797
  • Protein Target: Akt1, Akt2 and Akt3
  • Human Gene Name: AKT1, AKT2, AKT3
  • Mouse Gene Name: Akt1, Akt2, Akt3
  • Species Reactivity: mouse, rat, human, canine

PROMOTION TERMS

Free sample available. Limit 3 free samples for each full size Santa Cruz Biotechnology primary antibody ordered. All samples must be different. Customer responsible for shipping costs.