mouse monoclonal IgG2a; PGC-1α Antibody (D-5) is an IgG2a κ mouse monoclonal PGC-1 alpha antibody (also designated PPARGC1A antibody or PGC1A antibody) that detects the PGC-1 alpha protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PGC-1α Antibody (D-5) is available as both the non-conjugated anti-PGC-1 alpha antibody form, as well as multiple conjugated forms of anti-PGC-1 alpha antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Transcription factors exert their effects by associating with co-activator or corepressor proteins. The co-activator complexes are thought to be constitutively active, requiring only proper positioning in the genome to initiate transcription. Co-activators include the steroid receptor coactivator (SRC) and CREB binding protein (CBP) families that contain histone acetyltransferase (HAT) activity, which modifies chromatin structure. PPARgamma co-activator-1 (PGC-1) is a transcriptional cofactor of nuclear respiratory factor-1 (NRF-1), PPARbeta, PPARalpha and other nuclear receptors that is induced by exposure to cold temperatures and is involved in regulating thermogenic gene expression, protein uncoupling, and mitochondrial biogenesis. PGC-1 has a low inherent transcriptional activity when it is not bound to a transcription factor. Docking of PGC-1 to PPARgamma stimulates an apparent conformational change that then enables PGC-1 to bind to and assemble into complexes, which include the additional cofactors SRC-1 and CBP/p300, and results in a large increase in transcriptional activity.