E6874Hu
Human Transcriptional Coactivator Yap1, YAP1 ELISA Kit
Bioassay Technology Laboratory
DESCRIPTION
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097).
The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288).
Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702).
Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288).
The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).
Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).
In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity).
Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
DETAILS
- GeneID: 10413
- Species: Human
- Storage: 2-8ºC
- MASS(Da): 54,462
- Synonyms: COB1;Protein yorkie homolog;Transcriptional coactivator YAP1;YAP;YAP1;YAP2;YAP65;Yes-associated protein 1;Yes-associated protein YAP65 homolog;YKI
- Assay Time: 1h 30m
- Assay Type: Sandwich
- Gene Names: YAP1
- Reactivity: Human
- Sample Type: Serum, plasma, cell culture supernates
- Sensitivity: 0.093ng/ml
- Target Protein: YAP1
- Detection Range: 0.2-60ng/ml
- Full Product Name: Transcriptional coactivator YAP1
- Standard Solution: 64ng/ml
- UniProt Accession: P46937