DESCRIPTION

• Form: Culture supernatant
• Gene: GRIN1
• Host: Mouse
• Rrid: AB_2492165
• Type: Primary Antibody
• Clone: R1JHL
• Buffer: Lyophilized
• Target: NMDA NR1 Subunit
• Isotype: IgG
• Storage: Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
• Shipping: Blue Ice
• Synonyms: GluN1 antibody, Glutamate [NMDA] receptor subunit zeta-1 antibody, Glutamate receptor ionotropic N methyl D aspartate 1 antibody, Glutamate receptor ionotropic N-methyl-D aspartate subunit 1 antibody, glutamate receptor ionotropic NMDA 1 antibody, Grin1 antibody, MRD8 antibody, N methyl D aspartate receptor antibody, N methyl D aspartate receptor channel subunit zeta 1 antibody, N methyl D aspartate receptor subunit NR1 antibody, N-methyl-D-aspartate receptor subunit NR1 antibody, NMD-R1 antibody, NMDA 1 antibody, NMDA R1 antibody, NMDAR1 antibody, NMDA receptor 1 antibody, NMDA1 antibody, NMDAR antibody, NMDZ1_HUMAN antibody, NR1 antibody
• Clonality: Monoclonal
• Immunogen: Fusion protein containing amino acids 1-564 of the NR1 subunit of rat NMDA receptor.
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor.
• Applications: WB, IHC, ICC
• Ncbi Gene Id: 24408
• Conjugate Exem: Unconjugated
• Physical State: Lyophilized
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 120
• Production Notes: Culture supernatant
• Dilution Range Ip: 3 µg per 200 µg lysate
• Dilution Range Wb: 1:1000
• Immunogen Species: Rat
• Dilution Range Icc: 1:10
• Dilution Range Ihc: 1:400-1:1000
• Species Reactivity: Human, Mouse, and Rat
• Target Description: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to Nmethyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1-subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
• Dilution Range Wb Brain: 1:1000
• Uniprot Number Immunogen Species: P35439