DESCRIPTION

• Form: Lyophilized powder
• Host: Rabbit
• Clone: NA
• Label: Fluorescein isothiocyanate (FITC).
• Purity: Affinity purified on immobilized antigen.
• Target: Transmembrane Protein 119, Osteoblast induction factor, OBIF
• Comment: Contact Alomone Labs for technical support and product customization
• Gene Id: 231633
• Isotype: Rabbit IgG
• Homology: Rat - 12 out of 15 amino acid residues identical This antibody will not recognize human TMEM119
• Is Toxin: No
• Sequence: (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119 
• Synonyms: Transmembrane Protein 119, Osteoblast induction factor, OBIF
• Accession: Q8R138
• Clonality: Polyclonal
• Lead Time: 1-2 Business Days
• Reactivity: Rat|Mouse
• Formulation: PBS pH7.4, 1% BSA with 0.05% sodium azide
• Applications: FC
• Preservative: 1% BSA, 0.05% NaN3
• Reconstitution: 50 µl double distilled water (DDW).
• Blocking Peptide: BLP-NR175
• Negative Control: RIC-001-F
• Positive Control: NA
• Immunogen Location: Extracellular, N-terminus.
• Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
• Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
• Immunogen Source Species: Mouse
• Storage After Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
• Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
• Applications May Also Work In: FC
• Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
• Product Page - Scientific Background: Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.
• Standard Quality Control of Each Lot: Western blot analysis (unlabeled antibody, #ANR-175), and direct flow cytometry (labeled antibody).
• Application Dilutions Western Blot Wb: Contact Alomone for information
• Antibody Concentration After Reconstitution: 1 mg/ml
• Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: Contact Alomone for information