DESCRIPTION

• Aa: 285-593
• Mw: 60.3 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: M83738
• Species: Human
• Uniprot: P43378
• Shiptemp: -80°C (dry ice)
• Synonyms: MEG2, MEG-2, PTPN9
• Warnings: Avoid freeze/thaw cycles.
• Category: Protein Phosphatase/Non-Receptor
• Background: This PTP contains an N-terminal domain that shares a significant similarity with yeast SEC14, which is a protein that has phosphatidylinositol transfer activity and is required for protein secretion through the Golgi complex in yeast. This PTP was found to be activated by polyphosphoinositide, and is thought to be involved in signaling events regulating phagocytosis.
• References: 1. Q. Ying, et al., J. Cell Biochem. 2002, 86: 79.2. H. Huynh, et al. J. Immunol. 2003, 171: 6661.
• Description: Protein tyrosine phosphatase (GenBank accession number M83738), a.a. 285-593 (catalytic domain) with N-terminal GST-tag, MW=60.3 kDa, expressed in an E. coli expression system.
• Formulation: 25 mM Tris-HCl, pH 8.0, 67 mM NaCl, 1.4 mM KCl, 50% glycerol, 10 mM glutathione, and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and 2 ng/µl MEG-2. Assay was done at RT for 15 min, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
• Specific Activity: 1070 pmole/min/µg
• Storage Stability: >12 months at -80°C
• Scientific Category: PTP/Non-rec.