mouse monoclonal IgG1; HM74 Antibody (D-8) is an IgG1 κ mouse monoclonal HM74 antibody (also designated HM74 antibody) that detects the HM74 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. HM74 Antibody (D-8) is available as both the non-conjugated anti-HM74 antibody form, as well as multiple conjugated forms of anti-HM74 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. HM74, also known as PUMAG or Puma-g, is a member of the G protein coupled receptor (GPCR) superfamily. In humans, HM74 is encoded by two different genes (GPR109A and GPR109B) that produce proteins, namely HM74A and HM74 (or HM74B), which are 96% homologous. In mice and rats, only one gene encodes the HM74 protein (Gpr109a). HM74 is a G(i) protein-coupled receptor that mediates the metabolic effects of nicotinic acid. Localizing to the cell membrane, HM74 is highly expressed in adipocytes, immune cells and spleen. Like all members of the GPCR superfamily, HM74 contains seven transmembrane domains. HM74 lacks the N-linked glycosylation sites near the N-terminus that are present in other GPCR family members. Furthermore, HM74 shows a more diverged amino acid sequence homology from most family members, implying different ligand specificity.

Free sample available. Limit 3 free samples for each full size Santa Cruz Biotechnology primary antibody ordered. All samples must be different. Customer responsible for shipping costs.