Membrane, Nuclear, and Cytoplasmic Protein Extraction Kit: Membrane, Nuclear and Cytoplasmic Protein Extraction Kit is designed for efficient separation of membrane, nuclear and cytoplasmic fractions with little or no cross-contamination. The extracted membrane, nuclear and cytoplasmic proteins are functional and compatible with many downstream assays such as transcriptional activity, RNA splicing, gel shi assay, reporter assays, immunoprecipitation, enzyme activity assays, Western Blot, ELISA and so on. The kit offers a protease inhibitor cocktail and phosphatase inhibitor cocktail to maintain protein integrity and high biological activity. The kit is sufficient for 50X10^7 cells or 50x100mg tissue samples.