DESCRIPTION

• Application: FC, ICFC - Quality tested ICC - Verified
• Application Notes: Protocol: 1. Prior to reconstitution, place the vial to room temperature for a couple of minutes follow with quick Spin down the vial of lyophilized reagent vial. 2. Add 100 µl of DMSO to one vial of Fixable viability Dye - 405 and mix until fully dissolved. 3. Wash cells with PBS buffer (no Tris buffer and protein free). 4. Dilute Fixable viability Dye - 405 at 1:100 in PBS. Resuspend 1 million cells in diluted 100 µl Fixable viability Dye - 405 solution. To minimize background staining of live cells, titrate the amount of dye and/or number of cells per 100 µl for optimal performance. Different cell types can have a wide degree of variability in staining based on cell size and degree of cell death. Note: Don’t use Tris buffer as a diluent and be sure that the PBS does not contain any other protein like BSA or FBS. Note: The amount of dye used can also influence the ability to detect apoptotic as well as live and dead cells. 5. Incubate the cells at room temperature, in the dark, for 15-30 minutes. 6. Add 20 µl of Flow staining buffer (Cat# 102096) or 5 µl of 5% BSA. 7. Continue performing antibody staining procedure as desired. 8. Cells can be fixed with paraformaldehyde or methanol prior to permeabilization or can be analyzed without fixation.
• Excitationemission: Ex 403 / Em 427 nm
• Storage and Handling: • Store at -20°C upon receipt. Do not open vials until needed. • Once the DMSO is added to the Fixable Viability Dye - 405, use immediately, or store at -20°C in a dry place and protected from light, preferably in a desiccator or in a container with desiccant for no more than one month.