Anti-EGFR (Ser-1142), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: EGFR
Host: Rabbit
Rrid: AB_2096432
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: EGFR (Ser-1142)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: EGF, Epidermal, ErbB
Clonality: Rabbit Polyclonal
Immunogen: Phospho-EGFR (Ser-1142) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 1142 of human EGFR (ErbB-1). This human EGFR sequence has high homology with rat and mouse EGFR, and is not conserved in other ErbB family members.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody was cross-adsorbed to unphosphorylated EGFR (Ser-1142) coupled to agarose then affinity purified using phospho-EGFR (Ser-1142) peptide (without carrier). The antibody detects a 180 kDa* protein on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A, and does not detect this band after lambda phosphatase treatment.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 180
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:200
Species Reactivity: Human, Mouse, and Rat
Target Description: The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular ligand-binding domain and a cytoplasmic domain with intrinsic tyrosine kinase activity. The cytoplasmic domain has a C-terminal region with multiple autophosphorylation sites (Tyr-992, 1068, 1086, 1148, and 1173). These sites are important for downstream signaling and rapid internalization. In addition, EGFR activation leads to c-Src mediated phosphorylation of Tyr-845 and Tyr-1101. The former site is required for mitogenic responses to EGFR activation, while the latter may be an SH2 binding site. Phosphorylation of EGFR on serine and threonine residues is thought to represent a mechanism for regulation of receptor kinase activity and internalization. These sites include a PKC site (Thr-654), CAMKII sites (Ser-1046, 1047, 1057, and 1142), and constitutively phosphorylated sites (Ser-967 and Ser-1002). Thus, the regulation of EGFR activity involves a complex series of phosphorylation events at multiple sites throughout the intracellular portion of the receptor.
Uniprot Number Immunogen Species: P00533
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Ser-1142