Spike Trimer (S1+S2), His-tag, Eu-labeled (SARS-CoV-2) Recombinant

DESCRIPTION

Severe acute respiratory Coronavirus Spike trimer (S1+S2), Genbank Accession No. MN908947, a.a. 16-1213, with a with C-terminal His-tag, Eu-labeled, expressed in a HEK293 expression system. MW=139 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

DETAILS

Aa: 16-1213
Mw: 139 kDa +glycans
Tags: C-terminal His-tag
Format: Aqueous buffer solution.
Purity: >90%
Genbank: MN908947
Species: SARS-CoV-2
Uniprot: P0DTC2
Shiptemp: -80°C (dry ice)
Synonyms: covid, covid-19, covid19, coronavirus, Severe acute respiratory Coronavirus Spike trimer, SARS Cov-2 mutant, S protein, Spike S1, Spike S2, ncov
Warnings: Avoid freeze/thaw cycles.
Category: Coronavirus/Protein
References: 1. Klumpp-Thomas, C., et al., MedRxiv. 2020 Jul; preprint doi: 10.1101/2020.07.08.201473712. Yurkovetskiy, L., et al., bioRxiv. 2020 Jul; preprint doi: 10.1101/2020.07.04.187757
Description: Severe acute respiratory Coronavirus Spike trimer (S1+S2), Genbank Accession No. MN908947, a.a. 16-1213, with a with C-terminal His-tag, Eu-labeled, expressed in a HEK293 expression system. MW=139 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole and 20% glycerol.
Supplied As: Aqueous buffer solution.
Unspsc Code: 12352202
Unspsc Name: Proteins
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Product Type: Protein
Biosafety Level: Not applicable (BSL-1)
Assay Conditions: SARS-CoV2-Trimer Eu-labeled protein (6.25 nM) was incubated with ACE2, His-Avi-Tag, Biotin-labeled HiP (BPS Bioscience, #100665) and the Dye-labeled acceptor in ACE2-Spike TR-FRET buffer for 1 hour at room temperature. TR-FRET signals were measured and the ratio was calculated as [Ex340nm/Em665nm] /[Ex340nm/Em620nm].
Related Products: 100665, 100728, 79959, 100697
Storage Stability: At least 6 months at -80°C.
Scientific Category: Coronavirus