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K21007

MagVigen™- Anti-mouse IgG conjugates Kit

NVIGEN

DETAILS

  • Category: Purification Kit
  • Lead Time: 1 week
  • Subcategory: Magnetic Beads
  • Applications: Protein Purification, Co-immunoprecipitation
  • Product and Related Product Contents: Cat# 21007: MagVigen™-anti-Mouse antibody conjugates (Cat# 21006) are provided in phosphate buffered saline (PBS), pH 7.4. Each vial contains 1 ml of solution with a particle concentration of 1 mg/ml, which is enough for approximately 5-50 antibody enrichment or immunoprecipitation assays, or for binding 50 million cells. Washing Buffer (10X), 15 ml, Cat# A20001. Elution Buffer, 15 ml, Cat# A20002. All materials except Magnet should be stored at 4°C. Shelf life: 6 months

DESCRIPTION

Product Description MagVigen™-anti-Mouse Antibody are ideal for purification of mouse antibody tagged proteins or cells in immunoprecipitation assays or cellular assays. The subsequent nanoparticle bound proteins, protein complexes or cells can be easily separated from the rest of the sample by a magnet. The retained proteins can be eluted from the nanoparticles using an elution buffer. MagVigen™ nanoparticles enable identification of new protein- protein interactions through immunoprecipitation assays, where the MagVigen™ – protein complex can be used to isolate particular proteins of interest or protein complex from assay samples, e.g. cell lysate. The immunoprecipitated proteins can be further analyzed by electrophoresis, protein staining, and mass spectrometry. MagVigen™ nanoparticles are much smaller than conventional micro-beads, and they have optimal surface chemistry. These features allow for better accessibility of the nanoparticles to the antigenic epitope and for less disturbance to the native functions of proteins or protein-protein complexes. In addition, the surfaces of MagVigen™ nanoparticles are uniquely coated to reduce non-specific interactions with cellular proteins and other biomolecules. This feature allows for a more specific “pull down” of real protein complex targets.