DESCRIPTION

• Mw: 57.8 kDa
• Tag: Myc-DDK
• Accn: NM_001077701
• Type: Human Tagged ORF Clone
• Refseq: NM_001077701.3
• Status: In Stock*
• Symbol: MIER1
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 57708
• Orf Size: 1536 bp
• Synonyms: ER1; MI-ER1
• Refseq Orf: 1539 bp
• Uniprot Id: Q8N108
• Refseq Size: 5407 bp
• Availability: In Stock
• Cytogenetics: 1p31.3
• Gene Summary: This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal. [provided by RefSeq, May 2013]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC223015 representing NM_001077701 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCGGAGCCATCTGTTGAATCTTCAAGTCCAGGAGGTTCAGCAACATCAGATGACCATGAATTTGATC CATCAGCTGACATGCTGGTTCATGATTTTGATGATGAACGAACATTAGAAGAGGAAGAAATGATGGAAGG AGAAACAAACTTCAGCTCTGAAATAGAAGATCTTGCAAGGGAAGGCGACATGCCAATTCATGAACTTCTC AGCCTTTATGGTTATGGTAGTACTGTTCGACTACCTGAAGAAGATGAGGAAGAGGAAGAAGAGGAAGAAG AAGGTGAAGATGATGAAGATGCTGATAATGATGACAACAGTGGCTGTAGTGGGGAAAATAAAGAGGAGAA TATAAAGGATTCATCAGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGATCCATCACAATCTGTTGCT TCTCAAGATGCCCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAGTAGAAG AAGAATCTGAAGAAGATGAAGATTATATTCCATCAGAAGACTGGAAAAAGGAGATTATGGTGGGCTCCAT GTTTCAAGCAGAAATTCCAGTTGGCATTTGTAGATACAAAGAAAATGAAAAAGTATATGAAAATGATGAT CAGCTCCTGTGGGACCCTGAGTACTTACCAGAAGATAAAGTGATTATATTTCTTAAAGATGCATCTAGAA GAACAGGTGATGAGAAGGGTGTAGAAGCAATTCCTGAAGGATCTCACATAAAAGACAATGAACAGGCTTT ATATGAATTGGTTAAATGCAATTTTGATACAGAAGAAGCATTGAGAAGATTAAGATTTAATGTAAAAGCA GCTAGAGAGGAATTATCTGTTTGGACAGAGGAAGAGTGTAGAAATTTTGAACAAGGGCTGAAGGCCTATG GAAAGGATTTTCATTTGATTCAGGCTAATAAAGTCCGAACAAGGTCAGTTGGTGAATGTGTAGCATTCTA TTACATGTGGAAAAAATCTGAACGTTATGATTTCTTTGCTCAGCAAACACGATTTGGAAAGAAGAAATAT AATCTTCATCCTGGTGTAACGGATTACATGGATCGTCTTCTAGACGAAAGTGAAAGTGCTGCATCTAGTC GAGCACCATCCCCTCCCCCAACTGCATCAAACAGTAGTAACAGCCAGTCTGAGAAAGAAGATGGCACTGT AAGCACTGCTAATCAAAATGGAGTGTCATCTAATGGACCAGGTGAAATATTAAACAAAGAGGAAGTAAAA GTTGAAGGGTTACACATTAATGGACCAACAGGTGGAAATAAGAAACCACTTCATGCAGATATGGATACTA ATGGTTATGAAACAGATAACCTTACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGA TTTTGATGAAAAAAGTGAGAGACCTGCCAAAAGGCGAAGGGTAAACAGCAATGGAAAAGAAAGTCCAGGT TCTTCTGAATTTTTCCAAGAAGCAGTCTCACATGGGAAATTTGAAGAACTTGAAAACACAGATGAC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC223015 representing NM_001077701 Red =Cloning site Green =Tags(s) MAEPSVESSSPGGSATSDDHEFDPSADMLVHDFDDERTLEEEEMMEGETNFSSEIEDLAREGDMPIHELL SLYGYGSTVRLPEEDEEEEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSGQEDETQSSNDDPSQSVA SQDAQEIIRPRRCKYFDTNSEVEEESEEDEDYIPSEDWKKEIMVGSMFQAEIPVGICRYKENEKVYENDD QLLWDPEYLPEDKVIIFLKDASRRTGDEKGVEAIPEGSHIKDNEQALYELVKCNFDTEEALRRLRFNVKA AREELSVWTEEECRNFEQGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWKKSERYDFFAQQTRFGKKKY NLHPGVTDYMDRLLDESESAASSRAPSPPPTASNSSNSQSEKEDGTVSTANQNGVSSNGPGEILNKEEVK VEGLHINGPTGGNKKPLHADMDTNGYETDNLTTDPKLAHMTARNENDFDEKSERPAKRRRVNSNGKESPG SSEFFQEAVSHGKFEELENTDD TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin