DESCRIPTION

• Mw: 110 kDa
• Tags: none
• Format: Aqueous buffer solution
• Genbank: NM_033445, NM_003528, NM_003532, and NM_003548
• Species: human
• Shiptemp: -80°C (dry ice)
• Warnings: Avoid freeze/thaw cycles.
• Category: Methyltransferase/Substrates
• Background: The histone octamer is composed of a central heterotetramer of two copies of both histones H3 and H4, flanked by two heterodimers of histones H2A and H2B. The octamer assembles when the H3/H4 tetramer complexes with two H2A/H2B dimers. These histones of the histone octamer all contain N-terminal tails that emanate from their central histone folds and are targets for epigenetic modification.
• References: 1. Miller KM, Jackson SP. Biochem Soc Trans 40(2):370-6 (2012).2. Raut VV, Pandey SM, Sainis JK. Ann Bot 108(7):1235-46 (2011).3. Park K, Fasman GD. Biochemistry. 1987 Dec 15;26(25):8042-5.4. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20,5(1):11.
• Description: Human recombinant histone octamer consisting of 2 molecules each of histones H2A (a.a. 1-130(end)), H2B (a.a. 1-126(end)), H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Global MW = 110 kDa.
• Formulation: 45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Substrate for histone methyltransferase or acetyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases and acetyltransferases for drug discovery and HTS applications. Since the recombinant histone proteins in thi
• Product Type: Substrate
• Host Cell Line: E. coli
• Biosafety Level: Not applicable (BSL-1)
• Storage Stability: Stable at -80°C for at least 6 months from date of receipt, when stored as directed
• Scientific Category: Methyltransferase